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10103578001 in Ca 2 free Tyrode s solution 145 mM NaCl, 5 mM KCl, 10 mM HEPES, 5 mM NaHCO 3, 10 mM pyruvate, 10 mM glucose was injected under the lingual epithelium, which was then incubated for 15 min at 37 C priligy over the counter usa